Advanced Prep Stage
(≥ 24 hours)
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○ Spray internal surfaces of BSC with chemical sterilant ○ Expose internal surfaces of BSC to UV light ○ Prepare working surface of BSC with sterile drapes ○ Introduce sterile container for glutaraldehyde solution ○ Introduce non-sterile materials into glutaraldehyde solution
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Advanced Prep Stage
(≥ 10 hours)
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○ Remove material from glutaraldehyde and place in sterile packaging ○ Remove materials from BSC ○ Expose internal surfaces of BSC to UV light
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Pre-Surgery Prep Stage
(Day of Surgery)
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○ Assistant prepares working surface of BSC with sterile drapes using sterile technique ○ Surgeon dons cap, mask, surgical gown, and sterile gloves ○ Surgeon positions for surgery in BSC
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Animal Transport to
BSC
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○ Mice are place in sterile cage set-up inclusive of wire-bar and micro-isolator lid ○ Cages containing mice are placed into STSC ○ Inner port cap is secured within isolator ○ Transfer sleeve is removed from connection to outer transfer port of isolator and crimped ○ STSC is transported to BSC sash interface ○ Exposed surface of transfer sleeve is sprayed with chemical sterilant ○ Cages with mice are grasped by surgeon at sash interface and removed from STSC ○ Cages are positioned in the middle of the BSC working space
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Surgery Prep
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○ Surgical equipment is passed to surgeon using sterile technique at the BSC sash interface ○ Surgeon prepares fiber optic light at right of BSC sash interface with assistance ○ Surgeon prepares surgical work space for surgery
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Animal Transport to
Isolator
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○ Mice are transferred within BSC from recovery cage to sterile transport container ○ Sterile transport container is placed within sterile transport cage and handed out of BSC ○ Cage is transported to recovery isolator which has a sterile transfer port ○ Assistant wearing sterile gloves is prepared ○ External port cap is removed and transport cage lid is opened ○ Assistant wearing sterile gloves removes sterile transport container from cage ○ External surface of sterile transport container is sprayed with chemical sterilant ○ Sterile transport container is placed in isolator transfer port once sprayed ○ External isolator port cap is replaced and the transfer port sprayed with chemical sterilant ○ Mice are introduced into isolator and removed from transport container
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