. Author manuscript; available in PMC: 2015 Oct 21.

Published in final edited form as: Nat Commun. 2015 Sep 23;6:8342. doi: 10.1038/ncomms9342

Table 1.

Apparent blocker dissociation constants and kinetics

Blocker	MthK state	K_D^ap (μM)	k_on^ap (μM⁻¹ s⁻¹)	^*k_off^ap (s⁻¹)
TPeA	Open	4.6 ± 0.2	20 ± 3	92 ± 14
TPeA	Closed	2.1 ± 0.2	0.14 ± 0.02	0.29 ± 0.05
bbTBA	Open	2.1 ± 0.2	17 ± 2	35 ± 6
bbTBA	Closed	0.7 ± 0.2	0.018 ± 0.002	0.012 ± 0.004
BrbTBA	Closed	2.6 ± 0.4	0.028 ± 0.005	0.07 ± 0.02

k_off^ap = k_on^ap K_D^ap