Table 1.
Summary of SMRT sequencing and methylome analysis of representative strains containing the five modA alleles under study.
| modAallele | NTHi strain |
Clinical symptoms |
Accession number |
Methylation sequence |
Systematic name |
Number of sites in genome |
Genome size (bp) |
Predicted ORFs |
|---|---|---|---|---|---|---|---|---|
| modA2 | 723 | OM | CP007472 | 5′-CCGA(m6A)-3′ | M.Hin723I | 2,270 | 1,887,620 | 1,868 |
| modA4 | C486 | OM | CP007471 | 5′-CG(m6A)G-3′ | M.HinC486I | 6,203 | 1,846,507 | 1,783 |
| modA5 | 477 | OM | CP007470 | 5′-AC(m6A)GC-3′ | M.Hin477I | 2,548 | 1,846,259 | 1,813 |
| modA9 | 1209 | OM | JMQP01000000 | 5′-CCTG(m6A)-3′ | M.Hin1209I | 2,504 | 1,895,979 | 2,247 |
| modA10 | R2866 | Blood | CP002277* | 5′-CCT(m6A)C-3′ | M.Hin2866I | 1,244 | 1,932,238 | 1,905 |
*
Strain already annotated and submitted (October 2010) to the EMBL/GenBank/DDBJ databases. A full summary of SMRT sequencing/methylome analysis derived data is presented in Supplementary Fig. 1.