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Expression of mRNA in brain. RNA extracts from brains of AAV-DHCR7 treated (lanes 1-11) and saline treated (lanes 12-17) mice were tested for hDHCR7 mRNA (A) and mouse GAPDH mRNA (B). Non-reverse transcriptase treated samples showed the absence of hDHCR7 or GAPDH specific PCR product confirming that the amplification products result from mRNA derived cDNA rather than from the presence of contaminating DNA (data not shown).
