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. Author manuscript; available in PMC: 2016 Sep 23.
Published in final edited form as: Arthritis Rheumatol. 2016 Jan;68(1):127–137. doi: 10.1002/art.39337

Figure 1. Hedgehog signaling regulates expression of cholesterol homeostatic genes.

Figure 1

(A) Results from Affymetrix Human Gene 1.0 ST microarray of human osteoarthritic cartilage treated with Hh antagonist. Greater intensity of red represents increased gene expression with Hh inhibition. (B) Real-time PCR validation of Hh regulation of cholesterol homeostatic genes identified by microarray. Independent human osteoarthritic cartilage samples were treated identically to those used in microarray analyses. Expression in the control group was arbitrarily defined as ‘1’ (dashed line). Data from the groups treated with Hh antagonist (left) and Hh agonist (right) are given as the mean. Error bars are 95% confidence intervals (n = 3; *P < 0.05). (C) Western blot showing increased INSIG1 protein in extracts from human osteoarthritic cartilage explants treated with Hh antagonist (Treated). ACTIN is shown as a loading control.