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. Author manuscript; available in PMC: 2015 Nov 25.
Published in final edited form as: Nat Commun. 2015 Oct 9;6:8532. doi: 10.1038/ncomms9532

Figure 4. KCNJ15 knockdown abolished cathode as well as anode galvanotaxis.

Figure 4

(a) hTCEpi cells migrate toward cathode, and HaCaT and MDA-MB-231 cells migrate toward anode. Knockdown of KCNJ15 abolished directional migration in all three types of cells. At 48 h after transfection, cells were seeded onto galvanotaxis chamber. Positive directedness values indicate cathodal migration, whereas negative directedness value indicates anodal migration.

(b) KCNJ15 knockdown did not have significant effects on migration speed. hTCEpi (human corneal epithelial cells), HaCaT (human keratinocyte cells), and MDA-MB-231 (human breast cancer cells) were transfected with siRNA against KCNJ15 or control oligo. Cell numbers analyzed, 100 hTCEpi cells, 60 HaCaT cells, and 80 MDA-MB-231 cells. Results confirmed in two separate experiments. Statistical analysis was performed by the Student’s t-test. Data represented as mean ± SEM. *, p<0.05. **, p<0.01. NS, no significance. EF =200 mV mm−1.