Figure 2. Linear regression analysis of p53 expression in melanoma cell lines and IFN-γ production by cognate T cells.

Twelve of the 13 HLA-A2⁺ melanoma cell lines analyzed in Figure 1 and Table 1 were included with the 2 HLA-A2⁺p53⁺ tumor control lines (SaOS2/143 and MDA-231) in a linear regression analysis investigating the relationship between the specific MFI of p53 and IFN-γ production by the cognate effectors from Patient 1. Tumor cell line 624 was excluded from this analysis due to the close proximity of the amino acid change to the p53_264–272 epitope. This analysis is representative of results obtained with IFN-γ values by p53:264 TCR Td T cells from Patient 2 (R²=0.0013, p > 0.1) and the analysis performed upon excluding the effect from the outliers, MDA-231 and SaOS2/143.