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. Author manuscript; available in PMC: 2015 Sep 16.
Published in final edited form as: J Vis Exp. 2014 Sep 23;(91):51911. doi: 10.3791/51911

Figure 2. Aortic valve regions do not express ventricular myocardium markers.

Figure 2

Samples were prepared with increasing numbers of pooled embryonic mouse aortic valve regions, ranging from 1 to 4 valve regions per sample, or with a sample of the right ventricle. Samples were processed as described in Figure 1 and blotted for the ventricular myocardium markers ANP (A) and myosin light chain (MLC)-2v (B). ANP is completely restricted to the ventricular sample, and MLC-2v is expressed at extremely low levels compared with the ventricular sample. β-actin is shown as a loading control.