Gels showing limit of detection of rotavirus VP6 gene fragment (379 bp) by conventional RT-PCR using RNA or total nucleic acid extracted from a stool sample containing a high concentration of rotavirus (EIA OD value = 3.0) diluted 10−1 to 10−7 in a negative stool suspension, by each extraction method. A= MagNA Pure Compact, B= KingFisher Flex, C= QIAamp Viral RNA, D= miniMAG®, E= easyMAG® and F= RNaid®. MM= 100 bp Plus DNA Ladder.