. Author manuscript; available in PMC: 2015 Nov 2.

Published in final edited form as: Biochemistry. 2015 Oct 14;54(42):6501–6513. doi: 10.1021/acs.biochem.5b00881

Table 1. Catalytic Activities and Melting Temperatures of CdLS HDAC8 Mutants.


		Melting Temperature (T_m, °C)^a

	Activity^a (nmol product•μmol enzyme^-1•min^-1)	no ligand	with M344
wild type	1520 ± 90	50.1 ± 0.1^b	55.7 ± 0.2^b
H180R	0 ± 1	44.3 ± 0.3	44.1 ± 0.1
G304R	0 ± 1	43.3 ± 0.1	43.4 ± 0.1
G117E	70 ± 10	46.2 ± 0.1	49.2 ± 0.1
D233G	740 ± 40	43.3 ± 0.1	49.7 ± 0.1
P91L	1310 ± 60	49.3 ± 0.1	55.2 ± 0.1

All measurements made in triplicate and reported as mean ± standard deviation.

From reference 34.