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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: Cell Mol Gastroenterol Hepatol. 2015 Oct 13;2(1):63–76.e5. doi: 10.1016/j.jcmgh.2015.08.008

Figure 4. The impact of DSS induced colitis on p53 and apoptotic cells in Ugt1ΔIEC mice.

Figure 4

Ugt1ΔIEC and Ugt1F/F mice were treated with 3% DSS in drinking water for 5 days to induce acute colitis and IEC apoptosis. After another 5 days recovery with regular water, colon tissues were collected. (A) Expression of UGT1A, p53, p21, Bax, caspase-9, and caspase-3 detected by Western blot anaylsis. GAPDH was used as a loading control. Quantitation of each Western blot is presented in Supplemental Figure 2. (B) TUNEL staining of colons from untreated mice or DSS-treated mice (100× Magnification). At least four mice were analyzed in each group and the experiment was repeated twice. (C) Quantitation of the number of TUNEL positive cells in DSS-treated mice.