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. Author manuscript; available in PMC: 2015 Nov 10.
Published in final edited form as: J Neurosci. 2011 Jan 12;31(2):402–413. doi: 10.1523/JNEUROSCI.2897-10.2011

Figure 6.

Figure 6

Phosphorylation at Thr271 by Akt is necessary for survival-promoting activity of FoxG1.CGNs were transfected with plasmids expressing two separate phosphomimetic forms of FoxG1, Flag-FoxG1T271D, and Flag-FoxG1T271E. The cultures were then switched to HK or LK media or LK media supplemented with 200 nM wortmannin (Wort) or 20 μM LY294002 (LY). Viability of transfected neurons was quantified after immunocytochemistry and normalized to the viability of GFP-transfected neurons in HK. Survival-promoting effect of Flag-FoxG1T271D and Flag-FoxG1T271E in LK medium cannot be inhibited by inhibition of the PI-3 kinase–Akt pathway. Columns compared are indicated with bars. ***p< 0.001.