Table 2.
Genes selected for single gene expression analysis.
| Gene group | Symbol | Gene name |
Description | Function and reference | Fluidigm assay IDb |
|---|---|---|---|---|---|
| Cell marker | PTPRCa | CD45 | Protein tyrosine phosphatase, receptor type C | Leucocyte marker | GEP00055840 |
| PECAM1a | CD31 | platelet endothelial cell adhesion molecule-1 | Adhesion molecular, inflammation [32] | GEP00056436 | |
| CD34a | CD34 | Hematopoietic Progenitor Cell Antigen | EC marker, inflammation [33] | GEA00011907 | |
| ENGa | CD105 | Endoglin | EC marker, angiogenesis [34] | GEP00056632 | |
| MCAMa | CD146 | Melanoma cell adhesion molecule | EC marker, inflammation [35–38] | GEP00056760 | |
| KDR | Flk1 | vascular endothelial growth factor receptor 2 | EC marker, angiogenesis [39,40] | GEA00012361 | |
| FLT1 | VEGFR1 | vascular endothelial growth factor receptor 1 | EC marker, migration [41] | GEP00055864 | |
| TIE1 | Tie1 | tyrosine kinase with Ig-like and EGF-like domains 1 | EC marker, Angiogenesis [39,42] | GEA00012787 | |
| THBD | – | Thrombomodulin | EC marker [43] | GEA00014984 | |
| VWF | vWF | Von Willebrand factor | EC marker, angiogenesis [44] | GEA00013832 | |
| TEK | Tie2 | tyrosine kinase with Ig-like and EGF-like domains 2 | EC marker, angiogenesis [39,40] | GEA00013803 | |
| ACTG2 | α-actin | Actin, gamma-enteric smooth muscle | VSMC marker | GEA00025197 | |
| EPHB2 | EphB2 | Ephrin type-B receptor 2 | Arterial EC marker | GEA00029202 | |
| EPHB4 | EphB4 | Ephrin type-B receptor 4 | Venous EC marker, angiogenesis [45] | GEP00059920 | |
| Angiogenesis | VEGFA | VEGF-A | Vascular endothelial growth factor | Angiogenesis [40,46,47] | GEA00012311 |
| TGFB1 | TGF-b1 | Transforming growth factor beta1 | Modulate angiogenesis [34] | GEA00007272 | |
| PCNA | PCNA | Proliferating Cell Nuclear Antigen | Proliferation marker [48] | GEA00012343 | |
| CAT | – | catalase | Oxidative stress & Proliferation [28,49] | GEA00023106 | |
| SGK1 | SGK | serum-glucocorticoid-induced protein kinase | Proliferation [50] | GEP00060290 | |
| ANGPT1 | – | angiopoietin-1 | angiogenesis [40,51] | GEA00013518 | |
| ANGPT2 | – | angiopoietin-2 | Angiogenesis [52] | GEP00057393 | |
| HIF1A | HIF-1α | Hypoxia-inducible factor 1-alpha | Angiogenesis [53,54] | GEA00012495 | |
| NR4A1 | TR3 | human orphan receptor TR3 | Proliferation [55] | GEA00023496 | |
| ALOX5 | 5-LO | 5-lipoxygenase | Proliferation [56] | GEA00028402 | |
| CD44 | – | Proliferation, angiogenesis [57,58] | GEP00056546 | ||
| ACE | – | Angiotensin-converting enzyme | Angiogenesis [59] | GEP00058643 | |
| Inflammation | IL6 | – | Interleukin 6 | Inflammation [60] | GEA00012521 |
| IL8 | – | Interleukin 8 | Inflammation [61] | GEA00012363 | |
| VCAM1 | VCAM-1 | vascular cell adhesion molecule 1 | Inflammation [32] | GEP00056408 | |
| ICAM1 | ICAM-1 | Intercellular Adhesion Molecule 1 | Inflammation[32] | GEP00056359 | |
| TBXAS1 | THA-2 | thromboxane synthase-A2 | Inflammation [62] | GEP00060291 | |
| NOS3 | eNOS | endothelial nitric oxide synthase | Oxidative stress, Inflammation [63,64] | GEA00032450 | |
| CCL2 | MCP-1 | monocyte chemoattractant protein 1 | Inflammation [61,65,66] | GEP00055652 | |
| SELP | – | P-selectin | Adhesion molecular, Inflammation [32] | GEA00030146 | |
| PTGS1 | COX-1 | Cyclooxygenase-1 | Inflammation [67] | GEA00027133 | |
| PTGS2 | COX-2 | Cyclooxygenase-2 | Inflammation [46] | GEA00007158 | |
| ECM remodeling | MMP2 | MMP-2 | matrix metalloproteinase-2 | ECM metabolism [22,53,68] | GEA00013719 |
| MMP9 | MMP-9 | matrix metalloproteinase-9 | ECM metabolism, inflammation [22] | GEA00013721 | |
| MMP14 | MMP-14 | matrix metalloproteinase-14 | ECM metabolism [68] | GEA00026567 | |
| SERPINE1 | PAI-1 | Plasminogen activator inhibitor-1 | ECM metabolism [69,70] | GEP00056400 | |
| TNF | TNF-α | Tumor necrosis factor-α | ECM metabolism, inflammation [68] | GEP00059924 | |
| ITGA7 | – | Integrin-α | ECM metabolism [71] | GEP00058254 | |
| TIMP1 | TIMP-1 | Tissue inhibitor of metalloproteinase 1 | ECM metabolism, inflammation [72] | GEA00007289 | |
| TIMP2 | TIMP-2 | Tissue inhibitor of metalloproteinase 2 | ECM metabolism, inflammation [73,74] | GEA00020949 | |
| FN1 | – | fibronectin | ECM metabolism [75] | GEA00007778 | |
| TNC | – | Tenasin-C | ECM metabolism [76] | GEA00031358 | |
| SCEL | – | sciellin | ECM metabolism [77] | GEA00031897 | |
| PPL | – | periplakin | ECM metabolism [77] | GEA00032646 |
a
Genes used in FACS.
b
Primer sequences for microfluidic qPCR can be traced by these company assay IDs.