Figure 4. Adoptive transfer of 1,25D3-treated CD8⁺ T cells into CD8-deficient recipients fails to induce AHR.

Recipient mice were sensitized (2 intraperitoneal, i.p.) and challenged (4 nebulizations, Neb) using secondary allergen challenge model and received no cells, CD8⁺ T cells differentiated in IL-2 alone (CD8) or in the presence of 100 nM (CD8+100 nM 1,25D3) or 1 μM 1,25D3 (CD8+1 μM 1,25D3). (a) Changes in airway resistance (RL) were measured in response to increasing concentrations of methacholine. (b) Cell composition in BAL fluid. Data (mean+SEM) are from two to three experiments with three to four mice per experiment. *p<0.05, **p<0.01, ***p<0.001; compared to sensitized and challenged CD8-deficient recipients which received no cells. General linear models were employed; pairwise comparisons were performed using t-tests derived from these models. #p<0.05, ##p<0.01, ###p<0.001 compared to sensitized and challenged CD8-deficient recipients which received CD8⁺ T cells differentiated in IL-2 alone, these p-values remained significant after correction for multiple comparisons (Benjamini-Hochberg⁵⁷ correction); p-values that did not reach the threshold p-value after adjustment for multiple comparisons are shown numerically.