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. Author manuscript; available in PMC: 2016 Mar 4.
Published in final edited form as: Nat Commun. 2016 Jan 11;7:10213. doi: 10.1038/ncomms10213

Figure 7. 1,25D3-mediated VDR recruitment to the Cyp11a1 promoter region is altered in CD8+ T cells after adoptive transfer into sensitized and challenged CD8-deficient mice.

Figure 7

CD8+ T cells were differentiated in vitro in IL-2 in the presence or absence of 1 μM 1,25D3. CD8+ T cells from the lungs were recovered after adoptive transfer into sensitized and challenged CD8-deficient mice (n=3 mice/group). qPCR was performed using five Cyp11a1 promoter specific primers covering seven VDR binding sites. Data were analyzed via the percent input methodology: (2^ (CT of total input – CT of specific IP)) ×100 and relative percent input ratios using CD8+ T cells stimulated with IL-2 as baseline.