Figure 4.
YC-1 stimulates HO-1 gene expression in a soluble guanylate cyclase (sGC) and protein kinase G (PKG)-independent manner. A. Effect of the sGC inhibitor ODQ (30μM) on YC-1 (10-100μM for 10 minutes)-mediated increases in intracellular cGMP concentration. B. Effect of the sGC inhibitor ODQ (30μM) or the PKG inhibitor KT5823 (5μM) on YC-1 (100μM for 24 hours)-mediated increases in HO-1 protein. C. Effect of the sGC inhibitor ODQ (30μM) or the PKG inhibitor KT5823 (5μM) on YC-1 (100μM for 24 hours)-mediated increases in HO-1 mRNA. D. Effect of the sGC inhibitor DT-2 (5μM) on YC-1 (100μM for 24 hours)-mediated increases in HO-1 protein. E. 8-Br-cGMP (0-500μM for 24 hours) fails to stimulate HO-1 protein expression. HO-1 protein and mRNA are expressed in arbitrary units (a.u.). Results are means ± SEM of 3-4 experiments. *Statistically significant effect of YC-1.