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. Author manuscript; available in PMC: 2016 Jan 2.
Published in final edited form as: J Alzheimers Dis. 2015 Oct 1;48(3):647–665. doi: 10.3233/JAD-150398

Figure 6. Altered lncRNAs are activity-dependent regulated and induced by β-amyloid.

Figure 6

A) qRT-PCR showing the relative expression of glial (GFAP and S100β) and neuronal markers (DCX and NFH) and LOAD-dysregulated lncRNAs in commercially available RNA extracted from human neurons and human astrocytes. On the Y axe is depicted the expression of the analyzed gene relative to the housekeeping gene β-ACTIN. Gene expression is normalized to astrocytes.

(B and C) Immunostaining of human NSCs after 21 days of differentiation in vitro. hNSCs can be differentiated into a mixed population of neural cells. Neurons are stained with bIII-Tubulin (B) and MAP2 (C), while astrocytes are stained with GFAP (B). In both images cell nuclei are stained with DAPI. qRT-PCR analysis showing gene expression changes 1h post KCl stimulation (D) and 48h post Aβ treatment (E) of hNSCs differentiated for 21 days in vitro. On the Y axe is depicted the expression of the analyzed gene relative to the housekeeping gene β-ACTIN. Gene expression is normalized to non-treated and DMSO-treated cells respectively. Error bars are S.E.M.; * denotes p values <0.05; ** denotes p values <0.01.