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. Author manuscript; available in PMC: 2016 Apr 11.
Published in final edited form as: Cancer Sci. 2016 Feb 10;107(3):359–368. doi: 10.1111/cas.12859

Figure 5.

Figure 5

Glycolipid carrying I-antigens play important roles in integrin-mediated AKT phosphorylation and migration of prostate cancer cells. (A) Functional blocking antibodies against α5 and β1 integrins significantly inhibited cell migration in DU145NC and DU145GCNT2KD4 cells. (B) AKT phosphorylation at serine 473 (p-S473) was inhibited by the AKT inhibitor VIII (10 mM), and cell migration was inhibited in DU145NC cells but not DU145GCNT2KD4 cells. (C) DU145NC cells were cultured with benzyl-α-GalNAc (BAG), DL-threo-1-Phenyl-2-palmitoylamino-3-morpholino-1-propanol hydrochloride (PPMP) or with dimethyl sulfoxide (DMSO) for 48 h. (C) DU145NC cells were cultured with DMSO or BAG on fibronectin-coated dishes for 20 min. Depletion of O-glycan had no effect on AKT p-S473. (D) BAG treated DU145NC cells had significantly inhibited cell migration. (E) Depletion of glycolipids in DU145NC cells significantly inhibited AKT p-S473 and (F) migration. Migration assays were conducted in triplicate.