| Paraformaldehyde |
Fisher |
T353-500 |
Make 10% solution first in de-ionized distilled water; make 4% with 1× PBS, adjust pH to 7.4 |
| 1× Phosphate buffered saline, pH 7.4 |
Invitrogen |
10010049 |
|
| 2% Bovine serum albumin (2% BSA) |
Sigma-Aldrich |
A3059-100g |
Dissolve 2 g BSA into 100 ml of 1× PBS |
| 0.2% Triton X100 in 2% BSA/PBS (blocking buffer) |
Sigma-Aldrich |
T9284-100mL |
Dissolve 0.2 ml/100 ml 2% BSA/PBS |
| 0.1 M Glycine |
Sigma-Aldrich |
G-7126 |
Add 0.185 g to 25 ml of 2% BSA/PBS |
| α-bungarotoxin |
Invitrogen |
T1175 |
Concentration 1:400 |
| SMI-312 |
Sternberger Monoclonals |
SMI312 |
Concentration 1:1,000 |
| SV2 |
Developmental Studies Hybridoma Bank |
SV2-Supernatant |
Concentration 1:10 |
| FITC goat anti-mouse IgG1 |
Roche |
3117731001 |
Concentration 1:100 |
| Silicone rubber |
Sylgard, Dow Corning |
Part # 184 |
Follow instructions that come with kit: can use multiple sized culture dish (30 mm, 60 mm, 100 mm) depending on needs |
| Vectashield fluorescent mounting medium |
Vector laboratories |
H-1000 |
This is not a hard-set medium. You will need to secure the cover slip with clear nail polish. |
| Small spring scissors |
Fine Science Tools |
15002-08 |
|
| Dissection forceps |
Fine Science Tools |
11295-51 |
|
| Software for CMAP recordings |
Scope 3.5.6; ADI |
|
|
| Disk surface electrodes |
Natus neurology |
019-409000 |
|
| Subdermal needle electrodes |
Natus neurology |
019-453100 |
|
| Conductive gel |
Aquasonic |
122-73720 |
|
| Stimulator/recording system for CMAP recordings |
ADI Powerlab 8SP stimulator |
|
|
| Amplifier for CMAP recordings |
BioAMP |
|
|
