Figure 4.
TdT-mediated dNTP nick end labeling (TUNEL) and Ki-67 staining in murine AVF at day 7 and 21 after placement of AVF in outflow vein alone (C) and MSC transplanted vessels (M). Upper panel is the representative sections from TUNEL staining at outflow vein of the M transplanted and C vessels at day 7. Brown staining nuclei are positive for TUNEL (arrow). Negative control is shown where the recombinant terminal deoxynucleotidyl transferase enzyme was omitted. Lower panel is representative sections after Ki-67 staining in outflow vessels removed mesenchymal stem cell transplanted vessels (M) or arteriovenous fistula alone (C) at day 7 after AVF placement. Brown staining nuclei are positive for Ki-67. IgG negative controls are shown.