Fig. 2.

STEP₆₁ contributes to the PCP-induced decrease in BDNF expression in cortical cultures. a Cortical neurons were infected with lentivirus containing luciferase vector (LV-Luc) or STEP shRNA (LV-shRNA) for 7 days, followed by control or PCP treatment (10 μM) for 24 h. b Cortical neurons from WT or STEP KO mice were treated with control or PCP (10 μM) for 24 h. c STEP₆₁ was added back to cortical neurons derived from STEP KO mice by infecting with AAV1/2 STEP₆₁ (AAV-STEP₆₁) for 7 days, followed by control or PCP treatment. Neurons were lysed after treatment and subjected to Western blotting. STEP₆₁ and its substrates were probed with phospho-specific- or pan-antibodies, and phospho-levels were normalized to total protein levels, and then to β-actin as loading control. Data were expressed as mean ± SEM, and statistical significance was determined using one-way ANOVA with post hoc Bonferroni’s test (*p < 0.05, **p < 0.01, n = 6 independent batches of cultures for a–c)