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. Author manuscript; available in PMC: 2016 Jun 22.
Published in final edited form as: Eur J Immunol. 2015 Dec 2;46(2):455–463. doi: 10.1002/eji.201545537

Figure 2.

Figure 2

IL-1β but not TNFα is required for dectin-1-induced TSLP secretion in mDC. (A) mDC were stimulated with CUR with or without IL-1β (10ng/ml) or TNFα (10ng/ml) for 24 hours (n=3). (B) mDC were pre-incubated for one hour with or without IL-1RA (1μg/ml) or anti-TNFα (5μg/ml) and were then stimulated with CUR (n=8), MP (n=11) or C. albicans for 24 hours (n=4). (C) mDC were stimulated with MP with or without IL-1β for 24 hours (n=9). (D) mDC were stimulated with CUR or MP for 2, 4, 8, 12 or 24 hours. (E–F) mDC were pre-incubated for one hour with or without caspase-1 (50μM) or caspase-8 (50μM) inhibitors and were then stimulated with CUR, MP or C. albicans for 24 hours (n=5). Cumulative data are displayed as mean ± SEM. TSLP and IL-1β was measured in 24 hour cell culture supernatants by ELISA. Statistical significance was calculated using one-way ANOVA with Bonferroni post-tests (*** p=0.001, ** p=0.01).