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. Author manuscript; available in PMC: 2016 Feb 16.
Published in final edited form as: J Am Chem Soc. 2015 Aug 25;137(35):11303–11311. doi: 10.1021/jacs.5b04366

Figure 10.

Figure 10

The RAm1-P1 fluorescent conjugate is a cellular client-based thermo-labile cellular proteostasis network capacity sensor in HEK293T cells. (a) P1 selectively binds to and reacts with RA and exhibits fluorescence only in HEK293T cells transfected with RA, as discerned from the confocal fluorescence images. (b) The confocal fluorescence images show that the pre-formed RAm1-P1 conjugate retained predominant solubility at 37 °C (first row), however aggregates predominated upon heating at 42 °C for 2 h (second row). Notably, reduction of the temperature from 42 °C to 37 °C for an additional 4 h after thermal stress eliminates the RAm1-P1 granular aggregate structures in the cell (third row), presumably as a con-sequence of the heat shock response transcriptional program-enabled refolding of RAm1-P1 and partial degradation of the RAm1-P1 conjugate (see main text). Images were taken using a Zeiss LSM710 confocal microscope.