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. Author manuscript; available in PMC: 2016 Feb 16.
Published in final edited form as: J Am Chem Soc. 2015 Aug 25;137(35):11303–11311. doi: 10.1021/jacs.5b04366

Figure 3.

Figure 3

P1 labels the active site Lys-210 residue of RA chemoselectively. P1 (50 µM) completely labeled RA (5 µM) within 5 min at 25 °C, as shown by LC-ESI-MS. The conjugate mass was observed at 29847 Da (apo-RA: 29623 Da, P1: 225 Da) (top panels). Mutation of the active site Lys-210 residue to alanine eliminated the covalent labeling of RA by P1. The unmodified RA K210A mutant mass was observed at 29564 Da (bottom panels).