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RAm1 is a sensor of proteostasis network capacity inside living E. coli cells using solubility as an indicator. After the application of a thermal stress that leads to proteome misfolding and aggregation, RAm1 partitioned into an insoluble state in living E. coli cells after 10 min of a heat stress at 45°C, as revealed by an SDS-PAGE gel visualized by immunoblotting. S = soluble fraction, I = insoluble fraction, T = total protein. Trigger factor was used as a loading control.
