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. Author manuscript; available in PMC: 2016 May 23.
Published in final edited form as: Cell Death Discov. 2016 Jan 25;2:15069. doi: 10.1038/cddiscovery.2015.69

Fig. 3. Blebbishields rapidly internalize fusogenic membranes and mitotic cells fuse to each other using surface-exposed Golgi membranes.

Fig. 3

a, Live blebbishields internalized labelled surface membranes (arrowheads), but fixed blebbishields did not. Note the labelling is restricted to the surface (arrows). DIC, differential interference contrast. b, Nocodazole-arrested mitotic cells did not internalize surface membranes. c, Genetic labelling of Golgi membranes using Golgi-CFP revealed that they were exposed to the cell surface (arrows) during metaphase and telophase in untreated 293T mitotic cells and underwent fusion during telophase. CF, mitotic cleavage furrow; FF, fusion furrow. DIC, differential interference contrast. d, 293T mitotic cells were capable of fusion with blebbishields derived from RT4P cells. n, number of colonies.