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. Author manuscript; available in PMC: 2016 Apr 21.
Published in final edited form as: JCI Insight. 2016;1(2):e85817. doi: 10.1172/jci.insight.85817

Figure 6. Heme oxygenase-1 overexpression increases fusion and inhibits fission.

Figure 6

Expression of protein markers for mitochondrial fusion and fission was assessed in the hearts of WT mice and mice with cardiac-specific overexpression of heme oxygenase-1 (cs-HO-1) 14 days after treatment with vehicle or doxorubicin (DOX) by Western blot. Data are presented as protein density relative to β-actin. Black circles depict data from WT mice, and white circles depict data from cs-HO-1 mice. Expression of the fusion markers (A) mitofusin 1 (Mfn1) and (B) mitofusin 2 (Mfn2). Expression of the fission markers (C) dynamin-related protein 1 (DRP1) and (D) mitochondrial fission 1 (Fis1). Data are expressed in arbitrary units as an average ± SEM after normalization to β-actin. Original blots are shown in Supplemental Figure 2. P < 0.05, ANOVA and the Newman-Keuls post-test used to determine statistically significant changes; n = 5 per group.