Table 1.
Visual and quantitative results using laboratory preparations of orthopoxviruses.
| Vaccinia virus | Monkeypox virus | |||||||
|---|---|---|---|---|---|---|---|---|
| Visual IDa | Quantitative results (test 1 and 2 only) | Visual IDa | ||||||
| Test 1 | Test 2 | Test 3 | Test 4 | Test 5 | Peak areab,c | Test 1 | Test 2 | |
| 108 pfu/ml | Y | Y | Y | Y | Y | 2248 ± 446 | Y | Y |
| 107 pfu/ml | Y | Y | Y | Y | Y | 2346 ± 85 | Y | Y |
| 106 pfu/ml | Y | N | N | N | Y | 165 ± 69 | Y | Y |
| 105 pfu/ml | N | N | N | N | N | 18 ± 6 | N | N |
| 104 pfu/ml | N | N | N | N | N | 32 ± 9 | N | N |
| Crude VZV | N | N | – | – | – | 26 | – | – |
| HLF lysate | N | N | – | – | – | 22 | – | – |
| BSC-40 lysate | N | N | – | – | – | 26 | – | – |
a
Identified positive (Y) or negative (N) by two independent users 15 min after sample application.
b
Peak area measurements are defined in Section 2.3.1.
c
Average and standard deviation measurements, no cutoff was defined by the ESE reader.