In pressure volume loop software, assign channels for pressure and volume recordings. (A) Using hemodynamic catheter module, set volume setting to 5 RVU and 25 RVU and select both volumes, (B) Open option under the channel exhibiting volume recordings, (C) Select unit conversion and open “2 point calibration”, select “point 1” and assign as 5 RVU and select “point 2” and assign as 25 RVU, (D) Set pressure to 0 mm Hg and 25 mm Hg, (E) Open options under the channel exhibiting pressure recordings, (F) Select unit conversion and open “2 point calibration”, select “point 1” and assign as 0 mm Hg and select “point 2” and assign as 25 mm Hg. Accept assignments by selecting “OK”.