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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Cell Mol Life Sci. 2015 Jun 26;72(24):4849–4866. doi: 10.1007/s00018-015-1973-4

Fig. 6. Knockdown of Rab5a inhibits LPS-induced VE-cadherin internalization in HPMECs.

Fig. 6

HPMECs were transfected with NC siRNA or Rab5a siRNA and stimulated with 1 µg/ml LPS for 6 h, and then incubated with anti-VE-cadherin antibodies against the extracellular domain of VE-cadherin (VE-cadherin-N at 4 °C for 1 h. The VE-cadherin internalization was monitored by the uptake of external VE-cadherin antibodies at 37 °C and visualized in fixed cells using secondary fluorescent antibodies (red). The cells were also stained with Rab5a antibodies (green). Colocalization between VE-cadherin and Rab5a was displayed as yellow color in these overlays. Scale bar 7.5 µm. The photographs represent three independent experiments