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. Author manuscript; available in PMC: 2016 Dec 15.
Published in final edited form as: Cell Mol Gastroenterol Hepatol. 2016 May;2(3):340–357. doi: 10.1016/j.jcmgh.2016.01.006

Figure 5. PepT1 expression is upregulated during colon cancer in mice.

Figure 5

A, The colonic mRNA levels of PepT1 were quantified by qRT-PCR and normalized to mRNA levels of the ribosomal protein, 36B4. Values are mean ± SEM (n=8–9 per group). B, Immunohistochemical analysis of mouse PepT1 was performed on colonic tissue sections from wild-type (WT) mice treated with AOM (10 mg/kg body weight) plus two cycles of 2.5% DSS, or water alone. Microscopic images were taken at 20X magnification. C, Whole-colon lysates were used in Western blot analyses for mouse PepT1. β-Actin was detected as the loading control. **, p < 0.01 and ***, p < 0.001.