. Author manuscript; available in PMC: 2017 Jul 1.

Published in final edited form as: Biochem Biophys Rep. 2016 Jul 1;6:101–107. doi: 10.1016/j.bbrep.2016.03.006

Table 1.

Total protein yield, enzyme specific activities, and indices of functional integrity of mitochondria isolated without and with nagarse

	MITO−	MITO+	MITO+/MITO− (Fold Δ)	P Value
Total protein yield mg	0.386 ± 0.05	0.290 ± 0.03	0.75	0.02
Citrate synthase activity nmol·min⁻¹·mg⁻¹	827.4 ± 65.4	1232.9 ± 83.5	1.49	0.0001
State 3 J_O nmol·min⁻¹·mg⁻¹	77.6 ± 8.6	142.6 ± 16.8	1.84	0.003
State 4 J_O nmol·min⁻¹·mg⁻¹	22.5 ± 2.4	31.0 ± 3.5	1.38	0.01
State 3 J_P nmol·min⁻¹·mg⁻¹	384.9 ± 36.9	771.4 ± 83.4	2.00	0.002
RCR	3.6 ± 0.4	4.6 ± 0.4	1.30	0.002
ADP/O	2.5 ± 0.1	2.7 ± 0.1	1.09	0.02

Values are means ± SE; MITO+, mitochondria samples treated with nagarse; MITO−, mitochondria samples not treated with nagarse; total protein yield was calculated as the product of the protein concentration in the final mitochondrial suspension times the suspension volume; state 3 Jo, state 3 O2 consumption rate; state 4 Jo, state 4 O2 consumption rate; state 3 Jp, state 3 ATP production rate. RCR, respiratory control ratio, is State 3 (maximum) Jo divided by State 4 (resting) Jo; State 3 Jp is the product of State 3 Jo and the ADP/O ratio.