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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Am J Surg Pathol. 2016 Sep;40(9):1232–1242. doi: 10.1097/PAS.0000000000000659

Figure 4. Electron microscopy and high resolution light microscopy examination of sphingomyelin accumulation and clearance from Kupffer cells.

Figure 4

Panel A demonstrates the characteristic ‘fingerprint’ whorls of sphingomyelin (SM) present in Kupffer cells at baseline in patient 3. Electron dense lipofuscin (L) is also present. Inserts, panel A: Kupffer cell sphingomyelin is dark purple in modified toluidene blue sections (left) and red in lysenin affinity sections (right). Panel B demonstrates the clearance of sphingomyelin, with lipofuscin remaining (L). Inserts, panel B: This residual lipofuscin appears light blue in modified toluidene blue sections (left) and blue-green in lysenin affinity sections (right). EM scale bars = 1μm.