Increased synaptic AMPAR expression in the neuropil 60 min after TNFα. A-C, Comparison of DIC and laser scanning confocal microscopy reveals synaptophysin-lined structures in the neuropil, many of which have morphology typical of large dendrites (arrows). D-I, Representative three-dimensional projections of motoneurons illustrating dose-dependent increases in synaptic GluR1 in the neuropil after TNFα. J, K, Automated quantification through the confocal stack of each cell reveals a dose-dependent increase in total synaptic GluR1 (*p < 0.0001 from lowest dose; †p<0.05 from middle dose) but no significant change in GluR2 after TNFα (p > 0.05). Bars represent group means across >20,000 confocal sections (12 animals; n = 4 subjects per group). Error bars indicate SEM. See supplemental Figure 3, available at www.jneurosci.org as supplemental material, for higher-order interactions. Scale bar, 30 μm.