Figure 1. PDK1 expression in the rat brain.
A–D, PDK1 immunoreactivity in coronal sections through forebrains of rat pups at postnatal day 7 (P7). PDK1 was visualized using ABC/DAB immunohistochemistry. The specificity of PDK1 labeling was confirmed in control experiments, in which the primary antibody was replaced by the non-immune rabbit IgG (E, F). Intense PDK1 staining was found in neuron-like cells in the neocortical layers II–VI (A, C) as well as the pyramidal cell layer of the Ammon’s horn and granule cell layer of the dentate gyrus (B, D). Similar pattern of PDK1 immunoreactivity was observed at P21 (data not shown). CA, cornu amonis; DG, Dentate Gyrus; scale bars are as indicated.
