Table 1.
Characteristics of CLL samples before and after xenograftingA
| CLL sample (Sex) | Ig isotype | IGHV | IGHV mutation statusC | Cytogenetic abnormality defined by FISH | CD38% | No. of NSG recipients | NSG recipients with circulating T cells at sacrificeD | Cells with differentiated phenotype | Plasma Ig detected (IgM+IgG) | Days at assessment (no. of recipients in brackets) |
|---|---|---|---|---|---|---|---|---|---|---|
| 0515 (M) | IgM | 4-39 | U-CLL | del13q14.3 | 59 | 4 | Yes (3) | Present | Present | 38 (3) |
| No (1) | Absent | Absent | 38 (1) | |||||||
| 0854 (M) | IgM | 1-03 | M-CLL | del13q14.3 | 1.3 | 4 | Yes (4) | Present | Present | 50 (1), 62 (3) |
| No (0) | NA | NA | NA | |||||||
| 1083B (M) | IgM | 4-38-2 | U-CLL | del13q14.3; del11q23.3 | 62 | 5 | Yes (3) | Present | Present | 105 (3) |
| No (2) | Absent | Absent | 105 (2) | |||||||
| 1122B (M) | IgM | 3-09 | U-CLL | tri12 | 77 | 10 | Yes (10) | Present | Present | 29 (5), 34 (5) |
| No (0) | NA | NA | NA | |||||||
| 1164B (M) | IgM | 4-34 | M-CLL | none detected | 99 | 10 | Yes (7) | Present | Present | 29 (3), 34 (4) |
| No (3) | Absent | Absent | 29 (2), 34 (1) | |||||||
| 1279 (M) | IgM | 1-02 | U-CLL | del13q14.3 | 0.6 | 4 | Yes (4) | Present | Present | 34 (4) |
| No (0) | NA | NA | NA | |||||||
| 1301B (M) | IgM | 4-31 | U-CLL | tri12 | 88 | 3 | Yes (3) | Present | ND | 33 (2), 56 (1) |
| No (0) | NA | ND | NA | |||||||
| 1429 (M) | IgG | 3-48 | M-CLL | del13q14.3 | 0.5 | 3 | Yes (1) | Present | ND | 28 (1) |
| No (2) | Absent | ND | 28 (2) | |||||||
| 1435 (M) | IgM | 1-08 | M-CLL | none detected | 0.3 | 4 | Yes (2) | Present | ND | 33 (1), 46 (1) |
| No (2) | Absent | ND | 33 (1), 46 (1) | |||||||
| 1463 (F) | IgM | 3-21 | M-CLL | del13q14.3 | 88 | 10 | Yes (4) | Present | Present | 50 (4) |
| No (6) | Absent | Absent | 50 (6) | |||||||
| 1523B (F) | IgM | 3-48 | U-CLL | del13q14.3 | 20 | 5 | Yes (2) | Present | ND | 46 (2) |
| No (3) | Absent | ND | 33 (2), 46 (1) | |||||||
| 1539 (M) | IgM | 3-30 | M-CLL | del13q14.3; del17p13 | 0.5 | 5 | Yes (5) | Present | Present | 46 (5) |
| No (0) | NA | NA | NA | |||||||
| 1623 (M) | IgM | 2-70 | M-CLL | ND | 1.6 | 3 | Yes (3) | Present | ND | 32(1), 50 (1), 83 (1) |
| No (0) | NA | ND | NA | |||||||
A
Euthanasia performed at least 28 days after xenografting.
B
Mice also used for studies with euthanasia before day 28 (see Supplemental Table 2).
C
M-CLL if IGHV sequence is >2% different from most similar germline gene; U-CLL if IGHV sequence is ≤2% different from germline gene.
D
Yes – ≥10 T cells per μl in blood at euthanasia.
NSG, NOD/Shi-scid,γcnull.