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Cholangiocyte spheroids were loaded with rhodamine, treated with vehicle (DMSO) or biliatresone for 12 h, and imaged every 20 min. (a) Representative spheroids at the times indicated. (b) Quantification of rhodamine loss from spheroids over 12 h. N=17 (biliatresone) and 18 (DMSO), from 6 independent experiments. The time at which >50% of luminal signal was lost was scored. Mean ± SD shown, p<0.0001. Scale bar is 10 μm.
