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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: Hepatology. 2016 May 20;64(3):880–893. doi: 10.1002/hep.28599

Figure 7. Biliatresone decreases SOX17 and silencing Sox17 phenocopies the biliatresone effect.

Figure 7

(a) Sox17 mRNA expression in cholangiocytes in 2D culture treated with biliatresone or vehicle (DMSO) for 24 h. * p<0.05. (b) Western immunoblot of cholangiocytes in 2D culture treated with biliatresone or vehicle (DMSO) for 24 h. GAPDH loading control. (c) Cholangiocyte spheroids treated with siRNA for Sox17 or with scrambled RNA for 24 h, then stained for ZO1 and with DAPI. (d) Cholangiocyte spheroids treated with siRNA for Sox17 for the times indicated, then immunostained for F-actin (green) and cellular tubulin (red); DAPI nuclear stain (blue). (e) GSH levels in cholangiocytes in 2D culture treated with siRNA for Sox17 or scrambled RNA, p=0.98. Graphs are mean ± SD. Size bars, 10 μm. Representative pictures for c and d of 3 independent experiments with duplicate wells of spheroids.