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. Author manuscript; available in PMC: 2017 May 23.
Published in final edited form as: Clin Transl Sci. 2016 May 11;9(3):149–157. doi: 10.1111/cts.12399

Figure 5.

Figure 5

Effect of reduced cellular NAMPT expression on the pharmacological activity of MTX. A549 cells were treated with either siRNA targeting NAMPT mRNA or a scrambled control siRNA and evaluated after 96-hours for differences in (A) NAMPT expression by western blot analysis, (B) intracellular NAD levels, (C) cell viability measured as resorufin relative fluorescence units (RFU), and (D) sensitivity to the growth inhibitory effects of MTX. Results from a single experimental evaluation including three independent replicates are presented here along with the resulting mean±SD. The western blot is representative of three independent experimental evaluations and the MTX growth inhibitory response data is representative of four independent experimental evaluations.