Caco-2 cells were transiently transfected with non-silencing control (Nsi) or SPRY2 siRNA (50 nM, 72hr) or a mixture of SPRY2 siRNA and p21 siRNA (50nM each, 72hr). Transfected cells (10,000/well) were plated in a ninety six well plate, serum starved overnight, treated with EGF (10ng/ml) or vehicle for 48 hr and cell proliferation was assessed by MTT assay. Data represents means ± SD of three independent experiments, *p< 0.05 (one way ANOVA).