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. Author manuscript; available in PMC: 2016 Sep 20.
Published in final edited form as: Mol Carcinog. 2015 Aug 21;55(9):1355–1368. doi: 10.1002/mc.22379

Fig. 3. Silencing of p21 reverts the effect of SPRY2 downregulation on EGF-induced cell proliferation.

Fig. 3

Caco-2 cells were transiently transfected with non-silencing control (Nsi) or SPRY2 siRNA (50 nM, 72hr) or a mixture of SPRY2 siRNA and p21 siRNA (50nM each, 72hr). Transfected cells (10,000/well) were plated in a ninety six well plate, serum starved overnight, treated with EGF (10ng/ml) or vehicle for 48 hr and cell proliferation was assessed by MTT assay. Data represents means ± SD of three independent experiments, *p< 0.05 (one way ANOVA).