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. Author manuscript; available in PMC: 2016 Jun 3.
Published in final edited form as: ACS Infect Dis. 2016 Feb 10;2(4):281–293. doi: 10.1021/acsinfecdis.5b00143

Figure 2. 1536-well luciferase-based screening assay is higher-throughput and more sensitive than former 384-well HCI assay.

Figure 2

Pb-Luc IC50 values for the MMV validation set of antimalarials screened by the luciferase-based 1536-well assay and by the 384-well high-content imaging (HCI) assay are compared. Each data point represents a single antimalarial compound. The most active compounds in both assays are labeled. The assays generate Pb-Luc IC50 values that correlate very well with eachother, as demonstrated by an R2 value of 0.83, however, the luciferase-based assay resulted in IC50s roughly 10× lower than in the HCI assay.