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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Cell Mol Life Sci. 2016 Apr 25;73(20):3917–3933. doi: 10.1007/s00018-016-2232-z

Figure 5. Long-term effect of Ang-1 and VEGF on endothelial-barrier protection is reliant on Akt1-mediated TJ stabilization.

Figure 5

(A–B) Representative images of claudin-5 staining of ShControl and ShAkt1 HMEC monolayers after 30 min (short-term) treatment with 20 ng/ml VEGF or 50 ng/ml Ang-1, compared to PBS control, respectively. Quantification of the claudin-5 expression levels in HMEC monolayers following VEGF and Ang-1 treatment as analyzed using NIH-Image J software is provided in the right panels (n=10). (C–D) Representative images of claudin-5 staining of ShControl and ShAkt1 HMEC monolayers after 24 h (long-term) treatment with 20 ng/ml VEGF or 50 ng/ml Ang-1, compared to PBS control, respectively. Quantification of the claudin-5 expression levels in HMEC monolayers following VEGF and Ang-1 treatment as analyzed using NIH-Image J software is provided in the right panels (n=10). *P < 0.01. (Scale bars: 20 μM).