Table 3.
TCR sequences of clonally expanded CD4+ T cells in pSS SG tissue
| Exp clone ID |
No. repeats/ total |
Vα gene | Vα | N | Jα | Jα gene | 2nd V gene | Vα | N | Jα | 2nd Jα gene |
Vβ gene | Vβ | NDN | Jβ | Jβ gene |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 2A | 2/80 | TRAV29/DV5 | CAAS | DYG | GSTLGRLYF | J18 | TRAV23/DV6 | CAAS | *SLSIN | SGGGADGLTF | J45 | TRBV30 | CAW | SFSRA | GANVLT | J2-6 |
| 3A | 2/91 | TRAV24 | CA | SP | DTGRRALTF | J5 | TRBV10-2 | CAS | RKGAD | YQPQHF | J1-5 | |||||
| 3B | 2/91 | TRAV8-6 | CAVSD | R | QAGTALIF | J15 | TRBV12-3 | CASS | PPDRVG | TEAFF | J1-1 | |||||
| 4A | 2/95 | TRAV8-1 | CAVN | SG | NNNDMRF | J43 | TRBV2 | CASSEA | AAY | YGYTF | J1-2 | |||||
| 4B | 2/95 | TRAV35 | CAG | VR | GAQKLVF | J54 | TRBV18 | CASSP | WTA | NTGELFF | J2-2 | |||||
| 5A | 6/91 | ND | TRBV4-2 | CASS | RDGGY | TGELFF | J2-2 | |||||||||
| 5B | 2/91 | TRAV26-1 | CIVRV | V | TGRRALTF | J5 | TRBV20-1 | CSA | TTSTGG | NEQFF | J2-1 | |||||
| 6A | 4/50 | TRAV12-1 | CVVN | VKPG | DYKLSF | J20 | TRAV8-3 | CAV | AGE# | TGFQKLVF | J8 | TRBV6-2 | CASS | PDGA | NQPQHF | J1-5 |
| 6B | 2/50 | TRAV14/DV4 | CAMR | A | NRDDKIIF | J30 | TRBV5-1 | CASS | RDSGGS | SYEQYF | J2-7 | |||||
| 6C | 2/50 | TRAV9-2 | CALSD | G | SNYQLIW | J33 | TRBV6-5 | CA | TRDSA | TNEKLFF | J1-4 | |||||
| 7A | 3/115 | TRAV13-2 | CA | FLG | DTGRRALTF | J5 | TRBV15 | CATSRD | SLVALAGGR | NEQFF | J2-1 | |||||
| 7B | 2/115 | TRAV8-2 | CVV | GT | TGFQKLVF | J8 | TRBV19 | CASSID | GPGGLG | YGYTF | J1-2 | |||||
| 7C | 2/115 | TRAV12-2 | CAV | RG | NNNDMRF | J43 | TRBV7-6 | CASSL | QAVS | GELFF | J2-2 | |||||
| 7D | 2/115 | TRAV8-6 | CAVS | GGG | NNARLMF | J31 | TRBV18 | CASSPP | GQV | YGYTF | J1-2 | |||||
| 7E | 2/115 | TRAV8-4 | CAVSE | SG | AAGNKLTF | J17 | TRBV7-9 | CASS | PRDS | YEQYF | J2-7 | |||||
| 7F | 2/115 | TRAV8-6 | CAVR | A | AGGTSYGKLTF | J52 | TRBV14 | CASSQD | ASGRGA | DTQYF | J2-3 | |||||
| 7G | 2/115 | TRAV12-1 | CVVK | RA | GNMLTF | J39 | TRAV9-2 | CAL | # | GATNKLIF | J32 | TRBV4-3 | CASSQ | ERTDF | YGYTF | J1-2 |
| 7H | 2/115 | ND | TRBV7-2 | CASS | TQTGTS | YGYTF | J1-2 | |||||||||
| 7I | 2/115 | ND | TRBV2 | CAS | TRSPSSY | SNQPQHF | J1-5 | |||||||||
| 8A | 3/83 | TRAV16 | CAL | TS | GGYNKLIF | J4 | TRBV20-1 | CSA | LQGAGR | DTQYF | J2-3 | |||||
| 8B | 2/83 | ND | TRBV20-1 | CSARD | LNA | GYTF | J1-2 | |||||||||
| 8C | 2/83 | TRAV3 | CAVRD | T | DSSYKLIF | J12 | TRAV2 | CA | GGVM | NSGGY KVTF | J13 | TRBV25-1 | CASSE | GLAG | TQYF | J2-3 |
| 8D | 2/83 | TRAV12-3 | CA | SF | NTPLVF | J29 | TRAV26-1 | CIVRV | R | DDKIIF | J30 | TRBV7-2 | CASSLA | GVED | GYTF | J1-2 |
| 8E | 2/83 | TRAV20 | CAVQ | AIP | GGYQKVTF | J13 | TRBV24-1 | CAT | GEQ | NTGELFF | J2-2 | |||||
| 8F | 2/83 | TRAV29/DV5 | CAAS | AS | AGGTSYGKLTF | J52 | TRBV28 | CASS | TGGRN | SYEQYF | J2-7 | |||||
| 8G | 2/83 | TRAV12-1 | CVVN | PK | NSGGSNYKLTF | J53 | TRBV29-1 | CS | ALGQD | GYTF | J1-2 | |||||
| 9A | 4/114 | TRAV21 | CA | PRS | NAGKSTF | J27 | TRAV23/DV6 | CAAS | N | TSGTYKYIF | J40 | TRBV20-1 | CSA | LKPGTNH | EQYF | J2-7 |
| 9B | 3/114 | TRAV26-1 | CIVR | APH | QAGTALIF | J15 | TRBV2 | CA | GWTSGT | QETQYF | J2-5 | |||||
| 9C | 3/114 | TRAV4 | CLVGD | LG | GGSQGNLIF | J42 | TRBV24-1 | CAT | ERDSI | YGYTF | J1-2 | |||||
| 9D | 2/114 | TRAV3 | CAVR | VS | SGTYKYIF | J40 | TRBV10-3 | CAISD | PQGEG | GYTF | J1-2 | |||||
| 9E | 2/114 | TRAV17 | CATE | R | NTGFQKLVF | J8 | TRBV10-1 | CASR | DQG | TGELFF | J2-2 | |||||
| 9F | 2/114 | TRAV13-1 | CAA | S | FSDGQKLLF | J16 | TRAV17 | CATD | G# | SGGYNKLIF | J4 | TRBV7-9 | CASSLA | G | NQPQHF | J1-5 |
| 9G | 2/114 | TRAV23/DV6 | CAAS | VP | NTGNQFYF | J49 | TRAV3 | CAVR | GH | NNDMRF | J43 | TRBV5-6 | CASSL | GQGTTS | NYGYTF | J1-2 |
| 9H | 2/114 | TRAV20 | CAV | LPV | QGGKLIF | J23 | TRBV7-9 | CASS | MD | EKLFF | J1-4 | |||||
| 9I | 2/114 | TRAV24 | CA | HVTD | AGKSTF | J27 | TRBV20-1 | CSAS | LMGI | NQPQHF | J1-5 | |||||
| 10A | 11/113 | TRAV24 | HPE | GKLIF | J23 | TRAV8-1 | CAVN | PL | TGANNLFF | J36 | TRBV6-5 | CASSYS | RGR | ETQYF | J2-5 | |
| 10B | 10/113 | TRAV6 | CAL | APP | GGGNKLTF | J10 | TRAV13-1 | CAA | # | GGYQKVTF | J13 | TRBV3-1 | CASSQE | GRDS | SYEQYF | J2-7 |
| 10C | 4/113 | TRAV12-2 | CAV | RI | GGYQKVTF | J13 | TRBV3-1 | CASSQ | EGREGR | NTQYF | J2-3 | |||||
| 10D | 3/113 | TRAV17 | CA | PQ | TSGSRLTF | J58 | TRAV20 | CAVQ | ALAS | GANSKLTF | J56 | TRBV3-1 | CASSQE | GRQV | SYEQYF | J2-7 |
| 10E | 2/113 | TRAV17 | CAT | RG | FGNEKLTF | J48 | TRBV6-1 | CASSE | SGKGSR | ETQYF | J2-5 | |||||
| 10F | 2/113 | TRAV19 | CALSEA | R | GANSKLTF | J56 | TRBV11-3 | CASSL | WD | NEQFF | J2-1 |
Each expanded (Exp) clone ID corresponds to the graphical depiction for each subject shown in Figure 2. The complete T cell receptor (TCR) gene usage, complementarity-determining region 3 (CDR3) sequences, and the number of sequence repeats detected are listed. The presence of a pound sign or an asterisk in the CDR3 sequence indicates a frame shift or stop codon, respectively. ND, not determined.