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. Author manuscript; available in PMC: 2016 Jul 18.
Published in final edited form as: Angew Chem Int Ed Engl. 2016 Jun 3;55(30):8561–8565. doi: 10.1002/anie.201601984

Figure 3.

Figure 3

Validation of top hits on a chip assay. A) Western blot analysis of WT and sgRNA-modified MDA-MB-231 cells one week after infection. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the loading control. B–D) Comparisons of the input and the output cells before and after separation of WT and modified MDA-MB-231 cells at a flow rate of 75 μLmin−1. WT cells were stained with CellTracker Green CMFDA Dye and modified cells with CellTracker Red CMTPX Dye. Both bright-field and fluorescent images are presented (scale bar: 30 μm). E) After separation on the chip, the ratios of modified MDA-MB-231 cells to WT cells (B–D) were calculated. Error bars indicate the standard error of the mean (SEM; n=3). *: p values (p < 0.005) were determined by the Student t-test.