Skip to main content
. Author manuscript; available in PMC: 2016 Jul 18.
Published in final edited form as: Angew Chem Int Ed Engl. 2016 Jun 3;55(30):8561–8565. doi: 10.1002/anie.201601984

Figure 4.

Figure 4

Effect of STK4 on cell deformability and cytoskeletal distribution. A) Quantification of cell proportions at the outlet after separating an equal mixture of STK4 KO cells and cells expressing FLAG-Cas9 only. Error bars indicate SEM (n=3). B) Co-staining of F-actin and STK4 with Alexa Fluor 488 Phalloidin and anti-STK4 (red) antibodies (scale bar: 5 μm). C) Staining of F-actin and CK18 in WT, Cytochalasin D treated, and STK4 KO MDA-MB-231 cells (scale bar: 5 μm). D) Western blot analysis of Vimentin, CK18, and F-actin in STK4 KO MDA-MB-231 cells. GAPDH was used as the loading control.