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. Author manuscript; available in PMC: 2016 Jul 18.
Published in final edited form as: Angew Chem Int Ed Engl. 2016 Jun 3;55(30):8561–8565. doi: 10.1002/anie.201601984

Figure 5.

Figure 5

STK4 depletion drives MCF-10A cells to be more invasive. A) Co-staining of F-actin and STK4 in MCF-10A cells (scale bar: 5 μm). B) Western blot analysis of WT and sgSTK4-modified MCF-10A cells. C) Real-time PCR to measure gene expression in STK4 KO MCF-10A cells. Cells expressing FLAG-Cas9 only were used as the control. D) STK4 depletion promotes invasion of MCF-10A cells, as detected by a Matrigel-coated transwell invasion assay (scale bar: 2 mm). Quantification data is shown. E) Mammosphere formation in MCF-10A/Cas9 only and MCF-10A/STK4 KO cells (scale bar: 50 μm). Quantification data are shown. F) The ratio of CD44high/CD24low stem-like cells in MCF-10A expressing FLAG-Cas9 only and in STK4 KO MCF-10A cells.