Table 2.
Primer name | Sequence code (5’→3’) |
---|---|
GadBFw | AGCTCTCCATGGCGATAATGCATTCCAACATCAa |
GadBRv | AGCTCTAAGCTTTCAGTGATGGTGATGGTGATGGTGATGGTGATGGTGATGGTGATGGAAGCCGGAGACCCb |
GadB-T-Fw | CATGGGCGTGGCCTACACGc |
GadB-D-Fw GadB-K-Fw |
CCACGTGGCTGCCGCTTCGd CGGACACGCGTACGGTCTGe |
GadB-T-Rv | CGTGTAGGCCACGCCCATGf |
GadB-D-Rv | CGAAGCGGCAGCCACGTGGg |
GadB-K-Rv | CAGACCGTACGCGTGTCCGh |
GadB-XhoI-Fw | CAACTCGAGGGAAACAGAAGGTTTGACAGi |
GadB-BamHI-Fw | CTGGGATCCTCAGTGATGGAAGCCGj |
Bd_gadB_pL_4_L | GCCAAGGCCTTCTCTAAGTTC |
Bd_gadB_pL_4_R | CCACCTGATAGGCGGTCTC |
bglC_pL_6_L | TGGAAGGACGAGGTGACTGk |
bglC_pL_6_R | CTACGCGCTTCGGATCATk |
h-gabra1_pL_2_L | GGACAAACAGTAGACTCTGGAATTG |
h-gabra1_pL_2_R | TCAAGTGGAAATGAGTGGTCA |
h-gabrb2_pL_27_L | ATGGCACCGTCCTTTATGG |
h-gabrb2_pL_27_R | TGGGTACCTCCTTAGGTCCA |
h-gabbr1_pL_87_L | CATGGACGCTTATCGAGCA |
h-gabbr1_pL_87_R | GATCATCCTTGGTGCTGTCA |
h-gabbr2_pL_1_L | GACACCATCAGGTTCCAAGG |
h-gabbr2_pL_1_R | GATGCTGTAGAGAGGTAGGGAGA |
h-gabdh_pL_45_L | GAGTCCACTGGCGTCTTCAC |
h-gabdh_pL_45_R | TTCACACCCATGACGAACAT |
The underlined sequence corresponds to an NcoI site
The underlined sequence corresponds to an HindIII site
The underlined sequence corresponds to the introduced mutation site for Threonine.
The underlined sequence corresponds to the introduced mutation site for Aspartate.
The underlined sequence corresponds to the introduced mutation site for Lysine.
The underlined sequence corresponds to the introduced mutation site for Threonine.
The underlined sequence corresponds to the introduced mutation site for Aspartate.
The underlined sequence corresponds to the introduced mutation site for Lysine
The underlined sequence corresponds to an XhoI site
The underlined sequence corresponds to an BamHI site
B. breve NCIMB8807 strain specific primers have been described previously by Pokusaeva et al. (79)