Table 2.

Oligonucleotide primers used in this study

Primer name	Sequence code (5’→3’)
GadBFw	AGCTCTCCATGGCGATAATGCATTCCAACATCAa
GadBRv	AGCTCTAAGCTTTCAGTGATGGTGATGGTGATGGTGATGGTGATGGTGATGGTGATGGAAGCCGGAGACCCb
GadB-T-Fw	CATGGGCGTGGCCTACACGc
GadB-D-Fw GadB-K-Fw	CCACGTGGCTGCCGCTTCGd CGGACACGCGTACGGTCTGe
GadB-T-Rv	CGTGTAGGCCACGCCCATGf
GadB-D-Rv	CGAAGCGGCAGCCACGTGGg
GadB-K-Rv	CAGACCGTACGCGTGTCCGh
GadB-XhoI-Fw	CAACTCGAGGGAAACAGAAGGTTTGACAGi
GadB-BamHI-Fw	CTGGGATCCTCAGTGATGGAAGCCGj
Bd_gadB_pL_4_L	GCCAAGGCCTTCTCTAAGTTC
Bd_gadB_pL_4_R	CCACCTGATAGGCGGTCTC
bglC_pL_6_L	TGGAAGGACGAGGTGACTGk
bglC_pL_6_R	CTACGCGCTTCGGATCATk
h-gabra1_pL_2_L	GGACAAACAGTAGACTCTGGAATTG
h-gabra1_pL_2_R	TCAAGTGGAAATGAGTGGTCA
h-gabrb2_pL_27_L	ATGGCACCGTCCTTTATGG
h-gabrb2_pL_27_R	TGGGTACCTCCTTAGGTCCA
h-gabbr1_pL_87_L	CATGGACGCTTATCGAGCA
h-gabbr1_pL_87_R	GATCATCCTTGGTGCTGTCA
h-gabbr2_pL_1_L	GACACCATCAGGTTCCAAGG
h-gabbr2_pL_1_R	GATGCTGTAGAGAGGTAGGGAGA
h-gabdh_pL_45_L	GAGTCCACTGGCGTCTTCAC
h-gabdh_pL_45_R	TTCACACCCATGACGAACAT

^aThe underlined sequence corresponds to an NcoI site

^bThe underlined sequence corresponds to an HindIII site

^cThe underlined sequence corresponds to the introduced mutation site for Threonine.

^dThe underlined sequence corresponds to the introduced mutation site for Aspartate.

^eThe underlined sequence corresponds to the introduced mutation site for Lysine.

^fThe underlined sequence corresponds to the introduced mutation site for Threonine.

^gThe underlined sequence corresponds to the introduced mutation site for Aspartate.

^hThe underlined sequence corresponds to the introduced mutation site for Lysine

ⁱThe underlined sequence corresponds to an XhoI site

^jThe underlined sequence corresponds to an BamHI site

^kB. breve NCIMB8807 strain specific primers have been described previously by Pokusaeva et al. (79)