(A) Survival of BCG in Atg5 deficient and control macrophages after starvation (Gutierrez et al., 2004). Results are pooled from three independent experiments.
(B) Survival of female mice after infection with 100 T. gondii parasites expressing luciferase. These data were pooled from 4 independent experiments.
(C) Weight of mice in Fig. 1B over the course of T. gondii infection.
(D) Light emission from mice in Fig. 1B after injection of luciferin.
(E) Quantification of parasites in the indicated tissues using the methods and standard curve in Supplemental Figure S3. These data were pooled from 2 independent experiments. ** P<0.001, *** P<0.0001.
(F) Representative images of mice 8 days after infection with T. gondii. The full data set of which these are representatives is provided in Supplemental Figure S2.
(G) Survival of male mice after infection with 200 T. gondii parasites. This dose is higher than that used in female mice in 1B.
(H) Survival of mice after inoculation with 2×105 CFUs of L. monocytogenes.
(I) L. monocytogenes colony forming units in spleen or liver three days after infection. These data were pooled from at least three independent experiments (20 control mice and 19 ATG5flox/flox-Lyz-Cre mice).