(A) The proportion of macrophages containing at least one T. gondii parasite; *P <0.05, ***P<0.0001.
(B) The number of T. gondii parasite per vacuole in infected cells; ***P<0.0001. For (A) and (B) data were pooled from at least three independent experiments in which at least 210 cells were counted per condition.
(C) Representative immunofluorescence images of T. gondii-infected macrophages 20 hr after infection stained with anti-mouse F4/80 (red), anti-T. gondii (green), and DAPI (blue). Bar= 100 µm.